Severe Insulin Resistance and Moderate Glomerulosclerosis in a Minipig Model Induced by High-Fat/ High-Sucrose/ High-Cholesterol Diet

• LIU Yi

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University Medical College of Shaoguan University

• WANG Zongbao

  Department of Laboratory Animal Science, Nanhua University

• YIN Weidong

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

• LI Qinkai

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

• CAI Manbo

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

• ZHANG Chi

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

• XIAO Junxia

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

• HOU Hongjie

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

• LI Hongguang

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

• ZU Xiuhong

  Institute of Cardiovascular Research, School of Life Sciences and Technology, Nanhua University

Abstract

To develop a minipig model of type 2 diabetes that simulates the common manifestations of the metabolic abnormalities and resembles the kidney pathology of type 2 diabetes in the human population, male Chinese Bama minipigs were divided into 2 groups (5 in each) and fed with a control diet (CD) or high-fat/ high-sucrose/ high-cholesterol diet (HFSCD) for 5 months. The biochemical parameters of blood and urine, and the oral glucose tolerance test were monitored after the feeding program. The insulin resistance was estimated by the HOMA-IR index and the glucose elimination constant (K_G), and beta-cell function by the HOMA-beta index and the acute insulin response (AIR). Glomerulosclerosis index (GSI) was semi-quantitated by the degree of glomerular lesions in kidney sections stained with Masson trichrome. Extracellular matrix deposition in the kidney was examined by the protein expression of type IV collagen, connective tissue growth factor (CTGF) and matrix metalloproteinases 2 (MMP-2) using immunohistochemistry. Feeding HFSCD to minipigs markedly caused hyperglycaemia, hyperinsulinaemia and dyslipidaemia. HOMA-IR was significantly increased while HOMA-beta, AIR and K_G were obviously decreased in the HFSCD group compared with control group. Microalbuminuria, glucosuria and moderate glomerulosclerosis were exhibited in HFSCD-fed minipigs. The expression of type IV collagen and CTGF was elevated whereas that of MMP-2 was reduced in the kidneys of HFSCD group compared with the CD group. We concluded that feeding HFSCD to Chinese Bama minipigs for 5 months can induce humanoid type 2 diabetes and early-stage diabetic nephropathy, and accelerate extracellular matrix deposition and glomerulosclerosis.<br>

Journal

• Experimental Animals

  Experimental Animals 56 (1), 11-20, 2007

  Japanese Association for Laboratory Animal Science

Keywords

• diabetic nephropathy
• diabetogenic diet
• extracellular matrix
• minipig model
• type 2 diabetes

Details

• CRID: 1390001205044658176
• NII Article ID: 10018453361
• NII Book ID: AA11032321
• DOI: 10.1538/expanim.56.11
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL BIB ID: 8597925
• PubMed: 17283886
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8597925
• Web Site: http://www.jstage.jst.go.jp/article/expanim/56/1/56_1_11/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007202132
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed