Carbonic Anhydorase Isoenzyme I (CA-I) Concentration in Feces and Urine as a Temporary Marker of Occult Blood in Beagle Dogs

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  • IGUCHI Kouji
    Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University
  • KAWATO Katsuhito
    Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University
  • SEITA Tetsurou
    Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University
  • KURIBAYASHI Takashi
    Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University
  • SHIMADA Tokihiro
    Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University
  • MATSUMOTO Mariko
    Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University
  • YAMAMOTO Yutaka
    Farm Animal Clinic and Research Center Inc.
  • YAMADA Manabu
    Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima
  • YAMAMOTO Shizuo
    Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University

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Other Title
  • Carbonic Anhydorase Isoenzyme 1 (CA-1) Concentration in Feces and Urine as a Temporary Marker of Occult Blood in Beagle Dogs

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Abstract

This study was undertaken to investigate whether the concentration of carbonic anhydorase isoenzyme I (CA-I) in canine feces and urine is useful as a temporary marker of occult blood. Concentrations of CA-I were measured by enzyme-linked immunosorbent assay (ELISA). Fecal CA-I concentrations in 113 healthy beagle dogs (50 male and 63 female) of various ages ranged from 4.3 to 16.7 ng/g feces (mean; 7.0 ± 2.9 ng/g feces). One milliliter of blood from 3 healthy beagle dogs was found to contain 1,047, 1,062 and 1,150 μg CA-I. The fecal CA-I concentrations of dogs receiving intragastric infusions of autologous blood (10 ml) were very low. However, the fecal CA-I concentrations of dogs receiving infusion of autologous blood (5 ml) into the ascending colon were very high. Detection of fecal CA-I would be useful for identifying dogs with hemorrhaging of the large intestine. Of 55 urinary samples collected from healthy beagle dogs by catheter, chemical tests for occult blood were negative in 44, but CA-I concentrations ranged from 1.8 to 12.6 ng/ml (mean; 6.9 ± 5.4 ng/ml) by ELISA. The CA-I concentrations of the other 11 samples, which tested positive for occult blood on chemical testing, ranged from 41.2 to 525.0 ng/ml by ELISA. Although CA-I is not a specific marker of erythrocytes, CA-I may be used to detect occult blood in canine feces and urine until a specific immunological test kit using antibody for Hb is developed.<br>

Journal

  • Experimental Animals

    Experimental Animals 56 (1), 43-49, 2007

    Japanese Association for Laboratory Animal Science

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