Surface Plasmon Resonance Immunosensor for IgE Analysis Using Two Types of Anti-IgE Antibodies with Different Active Recognition Sites

• LI Yan

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• REN Jujie

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• NAKAJIMA Hizuru

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• SOH Nobuaki

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• NAKANO Koji

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• IMATO Toshihiko

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

Abstract

A simple and novel method for the determination of an IgE antibody based on a surface plasmon resonance immunosensor for the diagnosis of an allergy is described. The method involves the use of an anti-IgE(D) antibody and an anti-IgE(H) antibody, which reacts with the Ce2 domain and the Ce3 domain of the IgE antibody. The anti-IgE(D) antibody was immobilized on the gold surface of a sensor chip by physical adsorption. An IgE antibody sample was incubated by adding it to an anti-IgE(H) antibody solution to form an anti-IgE(H) immunocomplex through a reaction of the Ce3 domain of the IgE antibody. The incubated solution was introduced onto the sensor chip and the immunocomplex of the IgE-anti-IgE(H) then reacted with the anti-IgE(D) antibody immobilized on the sensor chip through the Ce2 domain of the IgE antibody part of the IgE-anti-IgE(H) immunocomplex. The detection limit of the present method for the determination of the IgE antibody was about 10 ppb. The affinity constants for the anti-IgE(H) antibody immunocomplex with the IgE antibody in solution and that of the anti-IgE(H) antibody immunocomplex with the IgE antibody immobilized on the sensor chip by a biotin-streptavidin interaction were estimated to be 4.1 × 10⁷ M^-1 and 5.8 × 10⁶ M^-1, respectively. The affinity constant for the immunocomplex of the anti-IgE(H) antibody with the IgE antibody with the anti-IgE(D) immobilized on the sensor chip was estimated to be 4.9 × 10⁷ M^-1, 20-times larger than the affinity constant for the IgE antibody immunocomplex with the anti-IgE(D) antibody immobilized on the sensor chip, based on a direct immunoassay method of the IgE antibody under the same experimental conditions.

Journal

• Analytical Sciences

  Analytical Sciences 23 (1), 31-38, 2007

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204255348480
• NII Article ID: 130004441364
• NII Article ID: 10018464003
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.23.31
• COI: 1:STN:280:DC%2BD2s%2Fhs1Kjtw%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 8598069
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8598069
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.23.31.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.23.31/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/23/1/23_1_31/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007175372
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed