Enzyme-based Field-Effect Transistor for Adenosine Triphosphate (ATP) Sensing

DOI PDF Web Site Web Site Web Site 被引用文献5件 参考文献38件
  • MIGITA Satoshi
    Department of Biological Functions and Engineering, Kyushu Institute of Technology
  • OZASA Kazunari
    RIKEN (The Institute of Physical and Chemical Research)
  • TANAKA Tomoya
    Department of Biological Functions and Engineering, Kyushu Institute of Technology
  • HARUYAMA Tetsuya
    Department of Biological Functions and Engineering, Kyushu Institute of Technology

この論文をさがす

抄録

Adenosine triphosphate (ATP) not only functions as an energy-carrier substance and an informative molecule, but also acts as a marker substance in studies of both bio-traces and cellular/tissular viability. Due to the importance of the ATP function for living organisms, in situ assays of ATP are in demand in various fields, e.g., hygiene. In the present study, we developed an ATP sensor that combines the selective catalytic activity of enzyme and the properties of an ion selective field effect transistor (ISFET). In this system, the ATP hydrolyrase, “apyrase (EC 3.6.1.5.)” is encased in a gel and mounted on a Ta2O5 ISFET gate surface. When the enzyme layer selectively catalyzes the dephosphorylation of ATP, protons are accumulated at the gate because the enzymatic reaction produces H+ as a byproduct. Based on the interfacial enzymatic reaction, the response from the ISFET is completely dependent upon the ATP concentration in the bulk solution. This device is readily applicable to practical in situ ATP measurement, e.g. hygienic usage.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 23 (1), 45-48, 2007

    社団法人 日本分析化学会

被引用文献 (5)*注記

もっと見る

参考文献 (38)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ