Carbon Felt-based Bioelectrocatalytic Flow Detectors: Highly Sensitive Amperometric Determination of Hydrogen Peroxide Using Adsorbed Peroxidase and Thionine
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- HASEBE Yasushi
- Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology
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- IMAI Ryosuke
- Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology
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- HIRONO Masaki
- Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology
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- UCHIYAMA Shunichi
- Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology
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Horseradish peroxidase (HRP) and thionine (TN) were co-adsorbed onto a porous carbon felt (CF), and the resulting HRP and TN-adsorbed CF (HRP-TN-CF) was successfully used as a working electrode unit of a novel bioelectrocatalytic flow detector for a highly sensitive amperometric determination of hydrogen peroxide (H2O2). Co-adsorbed TN was essential to enhance the cathodic peak current of H2O2, and the current responses of the HRP-TN-CF-based detector were much larger than those of the HRP-CF-based detector (without TN). When air-saturated 0.1 M phosphate buffer (pH 7.0) was used as a carrier at a flow rate of 3.9 ml/min, cathodic peak currents of H2O2 (sample injection volume, 200 µl) obtained at an applied potential of 0 V (vs. Ag/AgCl) increased linearly up to 50 µM with a detection limit of 0.1 µM. Repetitive 100 sample injection of 100 µM H2O2 induced no serious current decrease, and RSD was 0.41 to 1.21% (n = 100). The HRP-TN-CF retained 42% of its original activity after 8 days of storage in 0.1 M phosphate buffer at 4°C.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 23 (1), 71-74, 2007
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204255332096
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- NII論文ID
- 130004441372
- 10018464162
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BD2s%2Fhs1KisA%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 8598191
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可