Development of a Surface-Reaction System in a Nanoliter Droplet Made by an Ink-jet Microchip

• KUDO Yuki

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• NAKAHARA Takako

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• NAKAGAMA Tatsuro

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• SEINO Nobuko

  Fine Technology Components Department, Tokyo Factory, Fuji Electric Systems Co., Ltd.

• SHINODA Masaki

  Fine Technology Components Department, Tokyo Factory, Fuji Electric Systems Co., Ltd.

• UCHIYAMA Katsumi

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

Abstract

A surface-reaction system in a nanoliter water pool using an ink-jet microchip was developed. The reaction system in the nanodroplets formed on a poly(dimethylsiloxane) (PDMS) coated glass slide increased the diffusion-controlled reaction without using a nano-pump, specialized connector or highly sensitive detector. When nanoliter droplets were placed on the PDMS surface with a distance of 100 µm between them by the ink-jet microchip, the repeatabilities of the fluorescence intensity were 2.9% RSD (n = 7). The used ink-jet microchip had 4 different injection ports, and the distance between the ports was 0.995 mm. It was necessary to correct the distance in order to mix or dilute samples in a small droplet. The correction was successfully performed by moving the X-Y stage using inhouse-made software. A linear relationship was obtained between the Resorufin concentrations and the fluorescence intensity. We applied this system to an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin A (IgA), and observed a difference in the fluorescence intensity derived from the amount of IgA (blank, 6.25 ng/mL, 12.5 ng/mL). These results show the usefulness of the open-type micro-analytical systems proposed by us.

Journal

• Analytical Sciences

  Analytical Sciences 23 (1), 91-95, 2007

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679232057984
• NII Article ID: 130004441376
• NII Article ID: 10018464243
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.23.91
• COI: 1:STN:280:DC%2BD2s%2Fhs1KitA%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 8598224
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8598224
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.23.91.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.23.91/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/23/1/23_1_91/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed