Galactose Oxidase Immobilized on Silica in an Analytical Determination of Galactose-containing Carbohydrates

• KONDAKOVA Lyudmila

  Institute of Surface Chemistry of National Academy of Sciences of Ukraine

• YANISHPOLSKII Victor

  Institute of Surface Chemistry of National Academy of Sciences of Ukraine

• TERTYKH Valentin

  Institute of Surface Chemistry of National Academy of Sciences of Ukraine

• BUGLOVA Tat'yana

  D.K. Zabolotny Institute of Microbiology and Vyrology of National Academy of Sciences of Ukraine

Abstract

Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse and continuous modes of a substrate flowing through a column with an immobilized biocatalyst. The linear measurement ranges for galactose-containing carbohydrates concentrations were determined.

Journal

• Analytical Sciences

  Analytical Sciences 23 (1), 97-101, 2007

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204255346048
• NII Article ID: 130004441377
• NII Article ID: 10018464269
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.23.97
• COI: 1:STN:280:DC%2BD2s%2Fhs1KitQ%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 8598240
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8598240
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.23.97.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.23.97/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/23/1/23_1_97/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed