Introduction to Protein Crystallography-Inside of a Black Box-(4) Background of Structural Refinement: Refinement

  • TANAKA Nobuo
    Graduate school of Bioscience and Biotechnology, Tokyo Institute of Technology

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  • タンパク質結晶構造解析入門―ブラックボックスの中身―(4)構造精密化の裏側をのぞいてみよう  精密化
  • ニュウモン コウザ タンパクシツ ケッショウ コウゾウ カイセキ ニュウモン ブラックボックス ノ ナカミ 4 コウゾウ セイミツカ ノ ウラガワ オ ノゾイテ ミヨウ セイミツカ

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Abstract

The protein's structure has to be refined to elucidate its biological function exactly, after it is approximately determined with the X-ray crystallography. Mathematically the conjugate gradient method is rapid and convenient to the refinement, giving the restraint in the structure as a gathering of parts of standard value. It would be possible to do the high accuracy in atomic level, because the high-quality data can have been observed.

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