モジュラータンパク質の構造解析 : 受容体構造生物学における中心課題 [in Japanese] Structural Analysis of Modular Proteins : A Case of Integrin Head Fragment [in Japanese]
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Extracellular proteins and receptor ectodomain fragments remain to be challenging targets for X-ray crystallography, due to the difficulties associated with the recombinant production as well as their intrinsic interdomain flexibility. We have determined crystal structures of the α<SUB>IIb</SUB>β<SUB>3</SUB> integrin headpiece fragment in complex with therapeutic antagonists. Unconventional tricks exploited during the course of the crystallization, including usage of a carbohydrate processing-deficient cell line, a"clasping"tag, extensive protease polishing, and the use of Fab fragment to rigidify the complex, are described.
X-RAYS 48(6), 411-417, 2006-12-25
The Crystallographic Society of Japan