マイクロチップ電気泳動の生物学的解析への応用の可能性 Possibility of Microchip Electrophoresis for Biological Application

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著者

    • 片岡 正俊 KATAOKA Masatoshi
    • 産業技術総合研究所健康工学研究センター生体ナノ計測チーム Nano-Bioanalysis Team, Health Technology Research Center, National Institute of Advanced Industrial Science Technology (AIST)
    • 木戸 淳一 KIDO Jun-ichi
    • 徳島大学ヘルスバイオサイエンス研究部歯周歯内治療学分野 Department of Periodontolgy and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School
    • 篠原 康雄 SHINOHARA Yasuo
    • 徳島大学ゲノム機能研究センター遺伝子発現分野 Division of Gene Expression, Institute for Genome Research, The University of Tokushima

抄録

Microchip electrophoresis has recently attracted much attention in the field of nuclear acid analysis due to its high efficiency, ease of operation, low consumption of samples and reagents, and relatively low costs. Nucleic acid fragments are separated by capillary electrophoresis in a chip with microfabricated channels, with automated detection as well as on-line data evaluation. Microfabricated devices are forecast to be fundamental to the postgenome era, especially in the field of genetics and medicine. However, although there are many reports of the use of these instruments to evaluate standard DNA, DNA ladders, PCR products, and commercially available plasmid digests, little information is available their use with biological material. In this report, we showed the accuracy of sizing and quantification of endonuclease-digested plasmid DNA. We also showed the feasibility of on-microchip endonuclease treatment of plasmid DNA and sequential analysis as an additional application for DNA analysis. Furthermore, to evaluate the possibility of microchip electrophoresis for biological application, the results of the examination of blood sugar in human plasma and mitochondrial membrane potential were shown.

収録刊行物

  • 電気学会論文誌. C, 電子・情報・システム部門誌 = The transactions of the Institute of Electrical Engineers of Japan. C, A publication of Electronics, Information and System Society  

    電気学会論文誌. C, 電子・情報・システム部門誌 = The transactions of the Institute of Electrical Engineers of Japan. C, A publication of Electronics, Information and System Society 127(2), 222-226, 2007-02-01 

    The Institute of Electrical Engineers of Japan

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各種コード

  • NII論文ID(NAID)
    10018480563
  • NII書誌ID(NCID)
    AN10065950
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    03854221
  • NDL 記事登録ID
    8668583
  • NDL 雑誌分類
    ZN31(科学技術--電気工学・電気機械工業)
  • NDL 請求記号
    Z16-795
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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