Development of Two Stages Cock's Semen Extender for Room Temperature Storage at Laboratory

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A simple cock's semen dilutor (two-stage) with minimum ingredients was developed in the present study. The stage-1dilutor was aimed to reduce the motility and metabolism during storage and the stage-2 dilutor was to be added just prior to insemination to revive the motility to facilitate the entry of spermatozoa into sperm host gland. Initially, the semen was diluted 1 : 1 with the stage-1 dilutor having lower pH that reduced the motility and metabolism. Subsequently, after 24h, the diluted semen was mixed slowly with another dilutor stage-2 at 1 : 2 ratios to revive the motility of the spermatozoa to normal level. Phosphate buffer was the base for both storage (stage-1) and recovery (stage-2) dilutors with a pH of 6.5 and 7.5, respectively. In stage-1 dilutor sodium glutamate to minimize metabolism of spermatozoa and antibiotics to check bacterial growth were used whereas these were absent in stage-2 dilutor. Glucose was added as energy source in the dilutor of both stages. The osmolarity of stage-1 and stage-2 dilutors was 346 and 327Osm/kg H<SUB>2</SUB>0, respectively. This dilutor can be used to store the cock's semen at room temperature (17-21°C) for 24h with sustained motility. This new approach for two-stage dilutor of cock's semen storage at room temperature for extended period could be a useful A.I. tool under farm condition.

収録刊行物

  • The journal of poultry science  

    The journal of poultry science 44(1), 78-84, 2007-01-25 

    日本家禽学会

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各種コード

  • NII論文ID(NAID)
    10018485678
  • NII書誌ID(NCID)
    AA11564513
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13467395
  • NDL 記事登録ID
    8610996
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-J694
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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