Dynamic Analysis of the Developmental Fate of Cells in the Center of the Area Pellucida of the Blastoderm in Chicken

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The cells in the center of the area pellucida of the blastoderm have been used as donors for production of chimeric chickens, but the developmental fate of the cells has not been analyzed in detail. Also, little is known regarding the molecular mechanism of germline development and differentiation in early chick embryos. To clarify the developmental fate precisely, donor cells fluorescently labeled by PKH26 were analyzed in chimeric embryos. The cells were obtained from the center of the area pellucida of the donor and injected into the center of the area pellucida of the recipient. The dynamic states of the donor cells were tracked during development of the chimeric embryos, thereby allowing analysis of the developmental fate of the cells of the center of the area pellucida. The manipulated embryos that were injected with marked donor cells from the center of the area pellucida were cultured. The presence of the marked donor cells was confirmed in many embryos, through observation of embryos at each development stage under a fluorescence microscope. The results suggest that the migration system and developmental fate of cells from the center of the area pellucida are identical to those of primordial germ cells (PGCs). It was concluded that many PGCs or their precursor cells are localized in the center of the area pellucida of the blastoderm region.

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