Structure and Tissue Distribution of Cholecystokinin-1 Receptor in Chicken

• Ohkubo Takeshi

  Faculty of Agriculture, Kagawa University

• Shamoto Kensaku

  Faculty of Agriculture, Kagawa University

• Ogino Takeshi

  Faculty of Agriculture, Kagawa University

抄録

Chicken cholecystokinin-1 receptor (chCCK1R) cDNA was isolated from a chicken brain and tissue distribution of the chCCK1R mRNA was determined by RT-PCR. The chCCK1R is composed of 429 amino acid residues and showed approximately 73% and 50% identity with those of mammalian CCK1R and CCK-2 receptor (CCK2R) at the amino acid level, respectively. In phylogenetical analysis, the chCCK1R belonged to a cluster of CCK1R and CCK-CHR, another CCK receptor cloned from chicken, was placed into a cluster of CCK2R. By RT-PCR analysis, whilst both chCCK1R and CCK-CHR mRNAs were widely expressed in the chicken tissues, the expression pattern for each receptor was slightly different. Abundant chCCK1R mRNA was observed in multiple tissues, while CCK-CHR mRNA expression was dominantly observed in the brain and proventriculus in the chicken. Taken together, chCCK1R is a potent peripheral CCKR and CCK-CHR cloned and characterized previously is classified as CCK2R in the chicken. Furthermore, mRNA expression for chicken CCKRs is possible to be regulated by tissue specific manner and that may be associated with the diverse roles of CCK and gastrin in the chicken.

収録刊行物

• The Journal of Poultry Science

  The Journal of Poultry Science 44 (1), 98-104, 2007

  日本家禽学会

キーワード

• cDNA cloning
• chicken
• cholecystokinin
• receptor

詳細情報

• CRID: 1390001205206807424
• NII論文ID: 10018485748
• NII書誌ID: AA11564513
• DOI: 10.2141/jpsa.44.98
• ISSN: 13490486
• ISSN: 13467395
• NDL書誌ID: 8611109
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8611109
• Web Site: http://www.jstage.jst.go.jp/article/jpsa/44/1/44_1_98/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可