Population Polymorphism of Trypanosoma cruzi in Latin America indicated by Proteome analysis and by in vitro amastigote proliferation

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著者

    • SONE TOSHIO
    • Department of Medical Zoology, Saitama Medical School
    • YANAGI TETSUO
    • Department of Protozoology, Institute of Tropical Medicine, Nagasaki University
    • TADA ISAO
    • Department of Parasitology, Faculty of Medicine, Kyushu University
    • KIKUCHI MIHOKO
    • Department of Immunogenetics, Institute of Tropical Medicine, Nagasaki University

抄録

Nineteen stocks of Trypanosoma cruzi originating from several endemic countries for Chagas' disease in Central and South America were subjected to two-dimensional protein electrophoresis analysis. The presence or absence of a total of492polypeptide spots among19gel profiles was determined. The stocks were classified into three major distinctive groups derived from (I) Central America and the northern part of South America; (IIa) Central America and the northern part of South America; and (IIb) central and southern parts of South America, which showed perfect concordance with the previously reported classification based on isozyme and DNA sequence analyses. Late log phase of each epimastigote was inoculated to human cell lines WI-38and Hs224.T originating from the lung and muscle, respectively, and the number of trypomastigotes released was counted. The number of trypomastigotes from T. cruzi in group I released from the two cell lines was significantly higher than that in group III (p&It;0.05). The findings suggested that the phenetic distance appearing within the T. cruzi may, to some extent, be associated with the intracellular growth of T. cruzi, one of the characteristic features of growth found in the species.

Nineteen stocks of <I>Trypanosoma cruzi</I> originating from several endemic countries for Chagas' disease in Central and South America were subjected to two-dimensional protein electrophoresis analysis. The presence or absence of a total of492polypeptide spots among19gel profiles was determined. The stocks were classified into three major distinctive groups derived from (I) Central America and the northern part of South America; (IIa) Central America and the northern part of South America; and (IIb) central and southern parts of South America, which showed perfect concordance with the previously reported classification based on isozyme and DNA sequence analyses. Late log phase of each epimastigote was inoculated to human cell lines WI-38and Hs224.T originating from the lung and muscle, respectively, and the number of trypomastigotes released was counted. The number of trypomastigotes from <I>T</I>. <I>cruzi</I> in group I released from the two cell lines was significantly higher than that in group III (p0.05). The findings suggested that the phenetic distance appearing within the <I>T</I>. <I>cruzi</I> may, to some extent, be associated with the intracellular growth of <I>T</I>. <I>cruzi</I>, one of the characteristic features of growth found in the species.

収録刊行物

  • Tropical medicine and health  

    Tropical medicine and health 34(4), 167-174, 2006-12-01 

    日本熱帯医学会

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各種コード

  • NII論文ID(NAID)
    10018486912
  • NII書誌ID(NCID)
    AA11912846
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13488945
  • データ提供元
    CJP書誌  IR  J-STAGE 
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