Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2'-bipyridyl)ruthenium(II) Electrochemiluminescence Detection
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Capillary electrophoresis (CE) coupling with a tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)<sub>3</sub><sup>2+</sup>) electrochemiluminescence (ECL) detection technique was developed for the analysis of two <i>β</i>-blockers, atenolol (AT) and metoprolol (ME). The parameters that influence the separation and detection, including the buffer pH and concentration, the separation voltage, the detection potential and Ru(bpy)<sub>3</sub><sup>2+</sup> concentration, were optimized in detail. The calibration curve was linear over a concentration range of two or three orders of magnitude for the two <i>β</i>-blockers. The detection limits for AT and ME were 0.075 and 0.005 µM (<i>S/N</i> = 3). The relative standard deviations (<i>n</i> = 8) of the ECL intensity and the migration time were 2.65 and 0.22% for AT, 2.82 and 0.34% for ME, respectively. The proposed method was applied to determine AT and ME in spiked urine samples; satisfactory results were obtained.
- Analytical Sciences
Analytical Sciences 23(2), 183-188, 2007-02-10
The Japan Society for Analytical Chemistry