Validation of a Method for Predicting the Precision, Limit of Detection and Range of Quantitation in Competitive ELISA
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- CHOI Dong Hwan
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- KATAKURA Yoshio
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- MATSUDA Rieko
- National Institute of Health Sciences
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- HAYASHI Yuzuru
- National Institute of Health Sciences
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- HIROBE Masato
- Japan EnviroChemicals, Ltd.
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- GODA Yasuhiro
- Japan EnviroChemicals, Ltd.
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- NINOMIYA Kazuaki
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- SHIOYA Suteaki
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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The mathematical model for predicting the precision, limit of detection (LOD) and range of quantitation (ROQ) in a competitive enzyme-linked immunosorbent assay (ELISA) proposed by Hayashi et al. (Anal. Chem., 2004, 76, 1295) was validated. The model describes the relative standard deviation (RSD) of concentration estimates by the RSDs of pipetting volumes of analyte, enzyme-conjugated antigen, antibody and substrate solutions, and the standard deviation (SD) of inherent absorbances between the wells in an ELISA plate. For 6 kinds of direct competitive ELISA kits, the LOD and ROQ predicted by the model agreed well with those obtained by experiments with real samples. It was also confirmed that the model is applicable to the prediction of uncertainty that depends on the pipetting error of the viscous antiserum solution. The model was demonstrated to be useful for estimating the LOD and ROQ of competitive ELISA.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 23 (2), 215-218, 2007
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679235186816
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- NII論文ID
- 10018497741
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 8628894
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可