Spectrophotometric Determination of Uric Acid Based on Fading of o-Hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethyl-ammonium Complex
A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the <i>o</i>-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01 - 0.20 µg ml<sup>-1</sup>, with an effective molar absorptivity at 635 nm, the relative standard deviation being 6.5 × 10<sup>5</sup> dm<sup>3</sup> mol<sup>-1</sup> cm<sup>-1</sup> and 1.5% (<i>n</i> = 5). This method is about 20-times more sensitive than the conventional methods. The method was successfully applied to the assay of UA in human urine.
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 23(2), 223-226, 2007-02-10
The Japan Society for Analytical Chemistry