Reduced Lipopolysaccharide (LPS)-Induced Nitric Oxide Production in Peritoneal Macrophages and Inhibited LPS-Induced Lethal Shock in Mice by a Sugar Cane (Saccharum officinarum L.) Extract
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A sugar cane extract (SCE) has been found to have an immunostimulating effect in several animals. Lipopolysaccharide (LPS) is known to induce endotoxin shock <I>via</I> the production of inflammatory modulators such as tumor necrosis factor (TNF)-α and nitric oxide (NO). We examined in the present study the effects of SCE on the TNF-α and NO production in LPS-stimulated mice peritoneal cells and the endotoxin shock in mice. The supplementation of SCE to peritoneal macrophages cultured with LPS resulted in a significant decrease in NO production. All the mice injected intraperitoneally with LPS and <small>D</small>-galactosamine (LPS+GalN) died within 24 h. However, a peritoneal injection, but no intravenous or oral administration, of SCE (500–1,000 mg/kg) at 3 to 48 h before the LPS+GalN-challenge resulted in a significantly improved survival rate. These results suggest that SCE had a protective effect on LPS-induced endotoxin shock <I>via</I> one of possible mechanisms involving the suppression of NO production in the mouse peritoneal cavity.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(12), 2853-2858, 2006-12-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry