Immobilization of Lipase on Epoxy Activated (1→3)-α-D-Glucan Isolated from Penicillium chrysongenum
Access this Article
Search this Article
A water-insoluble linear (1→3)-α-<small>D</small>-glucan was isolated from <I>Penicillium</I> mycelia. Three kinds of epoxy-activated microspheres of this glucan were prepared as supports for <I>Candida</I> sp. lipase (EC18.104.22.168) immobilization. The highest immobilization yield was 36.4%. The specific activity was 26.85 U/mg, and only 4.1% of activity was lost in comparison with the free enzyme used for immobilization. The higher thermal stability, storage stability, and reusability of the immobilized lipase make it a potential candidate for wide application.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(12), 2883-2888, 2006-12-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry