Suppression of AGE Precursor Formation Following Unilateral Ureteral Obstruction in Mouse Kidneys by Transgenic Expression of α-Dicarbonyl/L-Xylulose Reductase
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Unilateral ureteral obstruction (UUO) of kidneys causes acute generation of carbonyl stress. By electrospray ionization/liquid chromatography/mass spectrometry (ESI/LC/MS) we measured the content of methyl glyoxal, glyoxal, and 3-deoxyglucosone in mouse kidney extracts following UUO. UUO resulted in elevation of these dicarbonyls in the obstructed kidneys. Furthermore, the accumulation of 3-deoxyglucosone was significantly reduced in the kidneys of mice transgenic for α-dicarbonyl/<small>L</small>-xylulose reductase (DCXR) as compared to their wild-type littermates, demonstrating 4.91±2.04 <I>vs.</I> 6.45±1.85 ng/mg protein (<I>P</I>=0.044) for the obstructed kidneys, and 3.68±1.95 <I>vs.</I> 5.20±1.39 ng/mg protein (<I>P</I>=0.026) for the contralateral kidneys. On the other hand, collagen III content in kidneys showed no difference as monitored by <I>in situ</I> hybridization. Collectively, DCXR may function in the removal of renal α-dicarbonyl compounds under oxidative circumstances, but it was not sufficient to suppress acute renal fibrosis during 7 d of UUO by itself.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(12), 2899-2905, 2006-12-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry