Characterization of a Unique Mutant lysE Gene, Originating from Corynebacterium glutamicum, Encoding a Product That Induces L-Lysine Production in Methylophilus methylotrophus

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<I>lysE24</I> is an allele of <I>lysE</I> encoding an <small>L</small>-lysine exporter of <I>Corynebacterium glutamicum</I>. The mutant gene is able to induce <small>L</small>-lysine production in <I>Methylophilus methylotrophus</I>. Although <I>lysE24</I> has a mutation in the middle of <I>lysE</I> that results in chain termination, the entire <I>lysE</I> locus, including the region downstream of the short open reading frame, is necessary for <small>L</small>-lysine production. We propose that separate polypeptides are synthesized from the <I>lysE24</I> locus due to reinitiation of translation utilizing an existing start codon beyond the site of the frameshift, and present evidence that translational coupling is required to form the functional <I>lysE24</I> product. In addition, expression of <I>lysE24</I> induces <small>L</small>-lysine production in another methylotroph, <I>Methylobacillus glycogenes</I>. These data suggest that the <I>lysE24</I> product is a split protein and that this curious feature might be a structure necessary for its functioning in certain obligate gram-negative methylotrophs.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(12), 2927-2934, 2006-12-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018523753
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8596458
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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