Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene of Sinorhizobium fredii USDA191 Required for myo-Inositol Utilization

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<I>Sinorhizobium fredii</I> USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 <I>idhA</I> gene encoding <I>myo</I>-inositol dehydrogenase, an enzyme necessary for <I>myo</I>-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In <I>Bacillus subtilis</I>, <I>myo</I>-inositol dehydrogenase catalyzes the first step of the <I>myo</I>-inositol catabolic pathway. Recently <I>iolE</I> was identified as the gene encoding 2-keto-<I>myo</I>-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-<I>myo</I>-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing <I>myo</I>-inositol. An <I>iolE</I> ortholog was cloned from USDA191. USDA191 <I>iolE</I> was expressed in <I>Escherichia coli</I> as a His<SUB>6</SUB>-tag fusion and purified to exhibit 2-keto-<I>myo</I>-inositol dehydratase activity. Inactivation of USDA191 <I>iolE</I> led to defective <I>myo</I>-inositol utilization. USDA191 <I>iolE</I> partially complemented a <I>B. subtilis iolE</I> deficient mutant. These results suggest that <I>S. fredii</I> USDA191 utilizes a <I>myo</I>-inositol catabolic pathway, analogous to that of <I>B. subtilis</I>, involving at least <I>idhA</I> and <I>iolE</I>.

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  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(12), 2957-2964, 2006-12-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018523881
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8596534
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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