Production of Isocyclomaltopentaose from Starch Using Isocyclomaltooligosaccharide Glucanotransferase

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Production of a novel cyclomaltopentaose cyclized by an α-1,6-linkage, [ICG5; <I>cyclo</I>-{→6)-α-<small>D</small>-Glc<I>p</I>-(1→4)-α-<small>D</small>-Glc<I>p</I>-(1→4)-α-<small>D</small>-Glc<I>p</I>-(1→4)-α-<small>D</small>-Glc<I>p</I>-(1→4)-α-<small>D</small>-Glc<I>p</I>-(1→}], from starch was performed using isocyclomaltooligosaccharide glucanotransferase (IGTase) derived from <I>Bacillus circulans</I> AM7. The optimal conditions for ICG5-production from partially hydrolyzed starch were as follows: substrate concentration, 1.0% (w/v); pH, 5.5; temperature, 45 °C; reaction time, 24 h, IGTase, 1.0 unit/g-dry solid (DS); isoamylase, 2,500 units/g-DS. The yield of ICG5 reached 25.9% under optimal conditions. ICG5-production was achieved from partially hydrolyzed starch using a crude enzyme preparation containing IGTase. Finally, ICG5 was obtained in a yield of 17.9% (99.3% purity, 2,681 g-DS). A digestive test with a human salivary amylase, an artificial gastric juice, a pancreatic amylase, and small intestinal enzymes showed that ICG5 was an indigestible oligosaccharide.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(12), 3013-3018, 2006-12-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018524105
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8596751
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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