Production of Isocyclomaltopentaose from Starch Using Isocyclomaltooligosaccharide Glucanotransferase

• WATANABE Hikaru

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• TAKAKURA-YAMAMOTO Rohko

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• KUROSE Mayumi

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• YOSHIDA Kenshi

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• OKU Kazuyuki

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• SAWATANI Ikuo

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• NISHIMOTO Tomoyuki

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• KUBOTA Michio

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• CHAEN Hiroto

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

• FUKUDA Shigeharu

  Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc.

抄録

Production of a novel cyclomaltopentaose cyclized by an α-1,6-linkage, [ICG5; cyclo-{→6)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→}], from starch was performed using isocyclomaltooligosaccharide glucanotransferase (IGTase) derived from Bacillus circulans AM7. The optimal conditions for ICG5-production from partially hydrolyzed starch were as follows: substrate concentration, 1.0% (w/v); pH, 5.5; temperature, 45 °C; reaction time, 24 h, IGTase, 1.0 unit/g-dry solid (DS); isoamylase, 2,500 units/g-DS. The yield of ICG5 reached 25.9% under optimal conditions. ICG5-production was achieved from partially hydrolyzed starch using a crude enzyme preparation containing IGTase. Finally, ICG5 was obtained in a yield of 17.9% (99.3% purity, 2,681 g-DS). A digestive test with a human salivary amylase, an artificial gastric juice, a pancreatic amylase, and small intestinal enzymes showed that ICG5 was an indigestible oligosaccharide.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (12), 3013-3018, 2006

  公益社団法人 日本農芸化学会

キーワード

• cyclomaltopentaose
• α-1,6-linkage
• <I>Bacillus circulans</I>
• glucanotransferase
• starch

詳細情報

• CRID: 1390282681453801088
• NII論文ID: 10018524105
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60404
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 8596751
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8596751
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60404
• 本文言語コード: en
• データソース種別:
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