Purification and Characterization of an α-Amylase of Pichia burtonii Isolated from the Traditional Starter "Murcha" in Nepal

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著者

    • MURA Kiyoshi
    • Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
    • OKADA Sanae
    • Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture
    • TOKUE Chiyoko
    • Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
    • ARAI Soichi
    • Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture

抄録

Among more than 20 yeast strains isolated from the traditional starter "murcha" in Nepal, we characterized a yeast that might be involved in saccharification. This strain, identified as <I>Pichia burtonii</I>, produced an extracellular amylolytic enzyme when cultured in the presence of starch in the medium. Since no amylase secreted by <I>P. burtonii</I> has yet been reported, we purified the enzyme and determined its N-terminal amino acid sequence. Together with the results of a hydrolyzing activity assay toward various substrates, it was found to be an α-amylase. The purified enzyme, named <I>Pichia burtonii</I> α-amylase (PBA), was a glycoprotein with an apparent molecular mass of 51 kDa. Enzyme activity was optimal at pH 5.0 at 40 °C. The enzyme retained 80% of its original activity after incubation under the optimal pH condition at 50 °C for 30 min. The activity was inhibited by metal ions such as Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP>, Hg<SUP>2+</SUP>, Al<SUP>3+</SUP>, and Zn<SUP>2+</SUP>.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(12), 3019-3024, 2006-12-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018524123
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8596767
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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