Stereoselective Reduction of Carbonyl Compounds Using the Cell-Free Extract of the Earthworm, Lumbricus rubellus, as a Novel Source of Biocatalyst
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We found the reducing activity toward carbonyl compounds in the cell-free extract of the earthworm, <I>Lumbricus rubellus</I>. The earthworm extract had a reducing activity for keto esters in the presence of NADH or NADPH as a coenzyme. The earthworm extract reduced ethyl 3-methyl-2-oxobutanoate to the corresponding alcohol with a high enantiomeric excess (91%, <I>R</I>-form) at 50 °C in the presence of NADH. In particular, ethyl 2-oxoheptanoate was exclusively reduced to the corresponding (<I>R</I>)-hydroxyl ester with a high enantiomeric excess (>99%).
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(12), 3077-3080, 2006-12-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry