Stereoselective Reduction of Carbonyl Compounds Using the Cell-Free Extract of the Earthworm, Lumbricus rubellus, as a Novel Source of Biocatalyst

• ISHIHARA Kohji

  Department of Life Science, Okayama University of Science

• NAKAJIMA Nobuyoshi

  Industry, Government, and Academic Promotional Center, Regional Cooperative Research Organization, Okayama Prefectural University

Bibliographic Information

Other Title

• Stereoselective Reduction of Carbonyl Compounds Using the Cell-Free Extract of the Ealthworm, Lumbricus rubellus, Novel Source of Biocatalyst
• Stereoselective Reduction of Carbonyl Compounds Using the Cell-Free Extract of the Earthworm,<i>Lumbricus rubellus</i>, as a Novel Source of Biocatalyst

Abstract

We found the reducing activity toward carbonyl compounds in the cell-free extract of the earthworm, Lumbricus rubellus. The earthworm extract had a reducing activity for keto esters in the presence of NADH or NADPH as a coenzyme. The earthworm extract reduced ethyl 3-methyl-2-oxobutanoate to the corresponding alcohol with a high enantiomeric excess (91%, R-form) at 50 °C in the presence of NADH. In particular, ethyl 2-oxoheptanoate was exclusively reduced to the corresponding (R)-hydroxyl ester with a high enantiomeric excess (>99%).

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (12), 3077-3080, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• earthworm
• biocatalyst
• keto ester
• stereoselective reduction
• cell-free extract

Details

• CRID: 1390001206477108736
• NII Article ID: 10018524334
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60408
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 8597043
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8597043
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60408
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed