Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway
A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(12), 3081-3083, 2006-12-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry