Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

Abstract

A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (12), 3081-3083, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• 3-dehydroquinate
• 3-dehydroshikimate
• intermediates in shikimate pathway
• shikimate

Details

• CRID: 1390001206477107456
• NII Article ID: 10018524354
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60414
• COI: 1:CAS:528:DC%2BD2sXktVKisw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 8597068
• PubMed: 17151445
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8597068
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60414
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed