Production of Lean Beef Containing a High Content of trans-10, cis-12 Conjugated Linoleic Acid by Feeding a High-Temperature-Micro-Time-Treated Diet with Extruded Soybean
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The effects were observed of feeding a high-temperature-micro-time (HTMT)-treated diet of high-quality extruded soybean (ESB) on <I>trans-10</I>, <I>cis-12</I> conjugated linoleic acid (CLA) incorporation in intra-muscular fat and on the lipid metabolism of twelve Holstein steers. The animals were randomly allocated to three groups, the control group being fed on a normal diet, and the other two groups being fed on a diet containing high-quality ESB with or without the HTMT treatment. The experimental period was four months. The content of total CLA in both the HTMT and non-HTMT-treated diets was higher than that in the control diet (<I>P</I><0.05). The concentration of <I>trans-10</I>, <I>cis-12</I> CLA was significantly higher in the HTMT-treated diet than in the non-treated diets at <I>P</I><0.05, and the HTMT-treatment decreased the intra-muscular fat content (<I>P</I><0.05). The expression of fatty acid synthase (FAS) and the plasma insulin-like growth factor-1 (IGF-1) level in the HTMT-treated group were lower than those in the control group (<I>P</I><0.05). Lipoprotein lipase (LPL) mRNA level in the adipose tissue was lower in the non-HTMT-treated group than in the control (<I>P</I><0.05). These results show that the HTMT treatment increased the level of <I>trans-10</I>, <I>cis-12</I> CLA in the intra-muscular fat. This increased level of <I>trans-10</I>, <I>cis-12</I> CLA may inhibit lipogenesis in the intra-muscular fat of Holstein steers.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(11), 2589-2597, 2006-11-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry