Characterization of an Exo-β-1,3-D-galactanase from Streptomyces avermitilis NBRC14893 Acting on Arabinogalactan-Proteins

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A gene belonging to glycoside hydrolase family 43 (GH43) was isolated from <I>Streptomyces avermitilis</I> NBRC14893. The gene encodes a modular protein consisting of N-terminal GH43 module and a family 13 carbohydrate-binding module at the C-terminus. The gene corresponding to the GH43 module was expressed in <I>Escherichia coli</I>, and the gene product was characterized. The recombinant enzyme specifically hydrolyzed only β-1,3-linkage of two <small>D</small>-galactosyl residues at non-reducing ends of the substrates. The analysis of the hydrolysis products indicated that the enzyme produced galactose from β-1,3-<small>D</small>-galactan in an exo-acting manner. When the enzyme catalyze hydrolysis of the arabinogalactan-protein, the enzyme produced oligosaccharides together with galactose, suggesting that the enzyme is able to accommodate β-1,6-linked <small>D</small>-galactosyl side chains. These properties are the same as the other previously reported exo-β-1,3-<small>D</small>-galactanases. Therefore, we concluded the isolated gene certainly encodes an exo-β-1,3-<small>D</small>-galactanase. This is the first report of exo-β-1,3-<small>D</small>-galactanase from actinomycetes.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(11), 2745-2750, 2006-11-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018525050
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8560736
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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