Characterization of an Exo-.BETA.-1,3-D-galactanase from Streptomyces avermitilis NBRC14893 Acting on Arabinogalactan-Proteins

• ICHINOSE Hitomi

  Food Biotechnology Division, National Food Research Institute

• KOTAKE Toshihisa

  Graduate School of Science and Engineering, Saitama University

• TSUMURAYA Yoichi

  Graduate School of Science and Engineering, Saitama University

• KANEKO Satoshi

  Food Biotechnology Division, National Food Research Institute

Bibliographic Information

Other Title

• Characterization of an Exo-β-1,3-D-galactanase from Streptomyces avermitilis NBRC14893 Acting on Arabinogalactan-Proteins
• Characterization of an Exo ベータ 1 3 D galactanase from Streptomyces avermitilis NBRC14893 Acting on Arabinogalactan Proteins
• Characterization of an Exo-β-1,3-<scp>D</scp>-galactanase from<i>Streptomyces avermitilis</i>NBRC14893 Acting on Arabinogalactan-Proteins

Abstract

A gene belonging to glycoside hydrolase family 43 (GH43) was isolated from Streptomyces avermitilis NBRC14893. The gene encodes a modular protein consisting of N-terminal GH43 module and a family 13 carbohydrate-binding module at the C-terminus. The gene corresponding to the GH43 module was expressed in Escherichia coli, and the gene product was characterized. The recombinant enzyme specifically hydrolyzed only β-1,3-linkage of two D-galactosyl residues at non-reducing ends of the substrates. The analysis of the hydrolysis products indicated that the enzyme produced galactose from β-1,3-D-galactan in an exo-acting manner. When the enzyme catalyze hydrolysis of the arabinogalactan-protein, the enzyme produced oligosaccharides together with galactose, suggesting that the enzyme is able to accommodate β-1,6-linked D-galactosyl side chains. These properties are the same as the other previously reported exo-β-1,3-D-galactanases. Therefore, we concluded the isolated gene certainly encodes an exo-β-1,3-D-galactanase. This is the first report of exo-β-1,3-D-galactanase from actinomycetes.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (11), 2745-2750, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• exo-β-1,3-<small>D</small>-galactanase
• glycoside hydrolase family 43
• arabinogalactan-protein (AGP)
• <I>Streptomyces avermitilis</I>

Details

• CRID: 1390001206477547776
• NII Article ID: 10018525050
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60365
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 8560736
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8560736
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60365
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed