Isolation of Protoplast from Aerial Mycelia and Fruit-Body Formation from Regenerated Mycelia in Pleurotus cornucopiae
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We have developed a simple and efficient method for protoplast isolation from <I>Pleurotus cornucopiae</I>. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(11), 2751-2753, 2006-11-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry