Isolation of Protoplast from Aerial Mycelia and Fruit-Body Formation from Regenerated Mycelia in<i>Pleurotus cornucopiae</i>
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- MIZOGUCHI Masahide
- Department of Biology, Faculty of Science and Engineering, Konan University
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- KITANO Satoshi
- Department of Biology, Faculty of Science and Engineering, Konan University
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- TAKAHASHI Wataru
- Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association
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- SUGAWARA Fuyuki
- Akita Prefecture Forest Technical Center
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- TANAKA Osamu
- Department of Biology, Faculty of Science and Engineering, Konan University
書誌事項
- タイトル別名
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- Isolation of Protoplast from Aerial Mycelia and Fruit-Body Formation from Regenerated Mycelia in Pleurotus cornucopiae
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We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (11), 2751-2753, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206477550592
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- NII論文ID
- 10018525071
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8560748
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可