Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production
NADP-Dependent shikimate dehydrogenae (SKDH, EC 220.127.116.11) was purified from <I>Gluconobacter oxydans</I> IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(11), 2786-2789, 2006-11-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry