Disruption of Malate:Quinone Oxidoreductase Increases<scp>L</scp>-Lysine Production by<i>Corynebacterium glutamicum</i>

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  • MITSUHASHI Satoshi
    Biofrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.
  • HAYASHI Mikiro
    Biofrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.
  • OHNISHI Junko
    Biofrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.
  • IKEDA Masato
    Biofrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University

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  • Disruption of Malate:Quinone Oxidoreductase Increases L-Lysine Production by Corynebacterium glutamicum

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Genomic analysis of a classically derived L-lysine-producing mutant, Corynebacterium glutamicum B-6, identified a nonsense mutation in the mqo gene, which encodes malate:quinone oxidoreductase (MQO). The effect of mqo disruption on L-lysine production was investigated in a defined L-lysine producer, C. glutamicum AHP-3, showing approximately 18% increased production. To explore the underlying mechanisms of the increase, the mqo-disrupted strain was analyzed from the viewpoints of redox balance, activities of membrane-bound dehydrogenases, and transcriptome. The intracellular [NADH]/[NAD] ratio in the strain remained unchanged. Also, there were no significant differences in the activities of the membrane-bound dehydrogenases examined. However, transcriptome analysis showed that some TCA cycle genes, such as acn, sucC, and sucD, were down-regulated in the strain. These results suggest that the loss of MQO activity down-regulates the flux of the TCA cycle to maintain the redox balance and results in redirection of oxaloacetate into L-lysine biosynthesis.

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