Reduced Immunogenicity of β-Lactoglobulin by Conjugating with Chitosan

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著者

    • Aoki Tomomi AOKI Tomomi
    • Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
    • Iskandar Silvia ISKANDAR Silvia
    • Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
    • TAKAHASHI Koji
    • Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
    • HATTORI Makoto
    • Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

抄録

Bovine β-lactoglobulin (β-LG) was conjugated with chitosan (CHS) by means of a water-soluble carbodiimide to reduce the immunogenicity of β-LG. Each β-LG-CHS conjugate was purified by ion-exchange chromatography and hydrophobic chromatography. The conjugation between β-LG and CHS was confirmed by SDS–PAGE, the isoelectric point of the conjugate being higher than that of β-LG. Two types of the β-LG-CHS conjugate were obtained with molar ratios of β-LG to CHS of 1:1 (F1) and 1:2 (F2). Structural analyses by fluorescence measurement, ELISA with monoclonal antibodies and retinol-binding activity indicated that the conjugates had almost maintained the native structure of β-LG. The antigenicity of the β-LG-CHS conjugates was similar to that of β-LG in C3H/He mice. Reduction of the immunogemicity of β-LG was achieved by conjugation with CHS. In particular, F2 showed very low immunogenicity. B cell epitopes of β-LG and the conjugates recognized in C3H/He mice were determined with 15-mer multi-pin peptide; the linear epitope profiles of the conjugates were found to be similar to those of β-LG, while the antibody response to each epitope was dramatically reduced. Conjugation of β-LG with chitosan was effective for reducing the immunogenicity of β-LG.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(10), 2349-2356, 2006-10-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018525416
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8523668
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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