Temperature Adaptation of Bacillus subtilis by Chromosomal groEL Replacement

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We investigated a temperature adaptation of <I>Bacillus subtilis</I> 168 in which chromosomal <I>groEL</I> was replaced with a psychrophilic <I>groEL</I>. This strain can grow at 50 °C but not at 51 °C, a temperature at which wild-type <I>B. subtilis</I> can grow. Using <I>in vivo</I> random mutagenesis by the <I>B. subtilis</I> mutator strain (<I>mutS</I>, <I>mutM</I>, <I>mutY</I>), two thermo-adaptants were isolated from the <I>groEL</I> substituted strain at 52 °C. They contained novel amino acid alterations in their ATP binding motif (T93I) and the inter-monomer contact (R285H) region of GroEL. These results suggest that GroEL participates in bacterial temperature adaptation.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(10), 2357-2362, 2006-10-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018525451
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8523676
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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