Temperature Adaptation of Bacillus subtilis by Chromosomal groEL Replacement
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We investigated a temperature adaptation of <I>Bacillus subtilis</I> 168 in which chromosomal <I>groEL</I> was replaced with a psychrophilic <I>groEL</I>. This strain can grow at 50 °C but not at 51 °C, a temperature at which wild-type <I>B. subtilis</I> can grow. Using <I>in vivo</I> random mutagenesis by the <I>B. subtilis</I> mutator strain (<I>mutS</I>, <I>mutM</I>, <I>mutY</I>), two thermo-adaptants were isolated from the <I>groEL</I> substituted strain at 52 °C. They contained novel amino acid alterations in their ATP binding motif (T93I) and the inter-monomer contact (R285H) region of GroEL. These results suggest that GroEL participates in bacterial temperature adaptation.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(10), 2357-2362, 2006-10-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry