Effects of Cadmium Stress on Growth, Morphology, and Protein Expression in Rhodobacter capsulatus B10
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- MOHAMED FAHMY GAD EL-RAB Sanaa
- Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University
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- ABDEL-FATTAH SHOREIT Ahmed
- Departmet of Biology, Faculty of Science, Assiut University
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- FUKUMORI Yoshihiro
- Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University
Bibliographic Information
- Other Title
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- Effects of Cadmium Stress on Growth, Morphology, and Protein Expression in<i>Rhodobacter capsulatus</i>B10
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Abstract
The effects of cadmium stress on growth, morphology, and protein expression were investigated in Rhodobacter capsulatus B10 using two-dimensional polyacrylamide gel electrophoresis and a scanning electron microscope with an energy dispersive X-ray spectrometer. The bacterium grew in the presence of 150 μM CdCl2 and highly induced heat-shock proteins (GroEL and Dnak), S-adenosylmethionine synthetase, ribosomal protein S1, aspartate aminotransferase, and phosphoglycerate kinase. Interestingly, the ribosomal protein S1 was proportionally expressed as the amount of cadmium in the medium, suggesting that S1 may be required for the repair of cadmium-mediated cellular damage. On the other hand, we identified five cadmium-binding proteins: 2-methylcitrate dehydratase, phosphate peripalsmic binding protein, inosine-5′-monophosphate dehydrogenase/guanosine-5′-monophosphate reductase, inositol monophosphatase, and lytic murein transglycosylase. The cadmium-treated cells had a filamentous structure and contained less phosphorous than the untreated cells. We propose that these characteristics of the cadmium-treated cells may be due to the inactivation of the phosphate peripalsmic binding protein and lytic murein transglycosylase by cadmium.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (10), 2394-2402, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681453888000
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- NII Article ID
- 10018525586
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 8523791
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed