Cloning, Expression, and Characterization of a Chitinase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9

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著者

    • Lan Xiqian LAN Xiqian
    • Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
    • Zhang Xin ZHANG Xin
    • Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
    • SHIMOSAKA Makoto
    • Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

抄録

The chitinolytic bacterium <I>Aeromonas hydrophila</I> strain SUWA-9, which was isolated from freshwater in Lake Suwa (Nagano Prefecture, Japan), produced several kinds of chitin-degrading enzymes. A gene coding for an endo-type chitinase (<I>chiA</I>) was isolated from SUWA-9. The <I>chiA</I> ORF encodes a polypeptide of 865 amino acid residues with a molecular mass of 91.6 kDa. The deduced amino acid sequence showed high similarity to those of bacterial chitinases classified into family 18 of glycosyl hydrolases. <I>chiA</I> was expressed in <I>Escherichia coli</I> and the recombinant chitinase (ChiA) was purified and examined. The enzyme hydrolyzed <I>N</I>-acetylchitooligomers from trimer to pentamer and produced monomer and dimer as a final product. It also reacted toward colloidal chitin and chitosan with a low degree of deacetylation. When cells of SUWA-9 were grown in the presence of colloidal chitin, a 60 kDa-truncated form of ChiA that had lost the C-terminal chitin-binding domain was secreted.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(10), 2437-2442, 2006-10-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018525764
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8523872
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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