Genes Encoding Small Heat Shock Proteins of the Silkworm, Bombyx mori
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Small heat shock protein (sHSP) is a family of ubiquitous polypeptides involved in a variety of physiological phenomena. From the silkworm, <I>Bombyx mori</I>, we isolated and sequenced the following cDNAs encoding sHSPs: <I>shsp19.9</I>, <I>shsp20.1</I>, <I>shsp20.4</I>, <I>shsp20.8</I>, <I>shsp21.4</I>, and <I>shsp23.7</I>. <I>shsp21.4</I> was nearly twice as large in size as other <I>shsps</I>. The deduced amino acid sequence of sHSP21.4 was similar to that of <I>Drosophila melanogaster</I> CG14207-PA. Other sHSPs were highly similar to each other and, in a phylogenetic tree, formed a cluster including <I>Plodia interpunctella</I> αCP25. It was speculated that <I>shsp21.4</I> has at least one intron in genome while other <I>shsps</I> do not. The transcripts of all <I>shsps</I> were subtle, but were constitutively detected in various tissues. Heat shock triggered a substantial increase in the transcripts other than <I>shsp21.4</I>. The <I>B. mori</I> sHSPs are perhaps classified into at least two groups: sHSP21.4 and others.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(10), 2443-2450, 2006-10-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry