Removal of p-Alkylphenols from Aqueous Solutions by Combined Use of Mushroom Tyrosinase and Chitosan Beads

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著者

    • KASHIWADA Ayumi
    • Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University
    • MATSUDA Kiyomi
    • Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University
    • HIRATA Mitsuo
    • Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University

抄録

Enzymatic removal of <I>p</I>-alkylphenols from aqueous solutions was investigated through the two-step approach, the quinone conversion of <I>p</I>-alkylphenols with mushroom tyrosinase (EC 1.14.18.1) and the subsequent adsorption of quinone derivatives enzymatically generated on chitosan beads at pH 7.0 and 45 °C as the optimum conditions. This technique is quite effective for removal of various <I>p</I>-alkylphenols from an aqueous solution. The % removal values of 97–100% were obtained for <I>p</I>-<I>n</I>-alkylphenols with carbon chain lengths of 5 to 9. In addition, removal of other <I>p</I>-alkylphenols was enhanced by increasing either the tyrosinase concentration or the amount of added chitosan beads, and their % removal values reached >93 except for 4-<I>tert</I>-pentylphenol. This technique was also applicable to remove 4-<I>n</I>-octylphenol (4NOP) and 4-<I>n</I>-nonylphenol (4NNP) as suspected endocrine disrupting chemicals. The reaction of quinone derivatives enzymatically generated with the chitosan's amino groups was confirmed by the appearance of peaks for UV–visible spectrum measurements of the chitosan films incubated in the <I>p</I>-alkylphenol and tyrosinase mixture solutions. In addition, 4-<I>tert</I>-pentylphenol underwent tyrosinase-catalyzed oxidation in the presence of hydrogen peroxide.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(10), 2467-2475, 2006-10-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018525864
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8523967
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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