Conversion of Capsular Polysaccharide, Involved in Pellicle Formation, to Extracellular Polysaccharide by galE Deletion in Acetobacter tropicalis
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<I>Acetobacter tropicalis</I> SKU1100 produces a pellicle-forming capsular polysaccharide (CPS), consisting of galactose, glucose, and rhamnose. We cloned the <I>galE</I> gene, a UDP-galactose synthesis gene, from <I>A. tropicalis</I> SKU1100 by PCR. A <I>galE</I>-disruptant was prepared and found not to produce CPS and thus not to form a pellicle under the static condition. Instead, the Δ<I>galE</I> mutant secreted an extracellular polysaccharide (EPS), which was purified and found to have a unique character, different from the original CPS.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(10), 2536-2539, 2006-10-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry