High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
3-Dehydroshikimate was formed with a yield of 57–77% from quinate <I>via</I> 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent <small>D</small>-glucose dehydrogenase (GDH) in the presence of excess <small>D</small>-glucose as an NADPH re-generating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(10), 2579-2582, 2006-10-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry