High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria

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3-Dehydroshikimate was formed with a yield of 57–77% from quinate <I>via</I> 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent <small>D</small>-glucose dehydrogenase (GDH) in the presence of excess <small>D</small>-glucose as an NADPH re-generating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(10), 2579-2582, 2006-10-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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被引用文献:  7件

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各種コード

  • NII論文ID(NAID)
    10018526303
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    09168451
  • NDL 記事登録ID
    8524736
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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