High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

Abstract

3-Dehydroshikimate was formed with a yield of 57–77% from quinate via 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent D-glucose dehydrogenase (GDH) in the presence of excess D-glucose as an NADPH re-generating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (10), 2579-2582, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• <I>Gluconobacter oxydans</I>
• NADP-shikimate dehydrogenase
• oxidative fermentation
• quinate oxidation
• shikimate production

Details

• CRID: 1390282681453735168
• NII Article ID: 10018526303
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60259
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 8524736
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8524736
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60259
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed