Tumor Antigen Occurs in N-Glycan of Royal Jelly Glycoproteins : Honeybee Cells Synthesize T-Antigen Unit in N-Glycan Moiety
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In our previous paper (Kimura, Y., <I>et al.</I>, <I>Biosci. Biotechnol. Biochem.</I>, <B>67</B>, 1852–1856, 2003), we found that a complex type <I>N</I>-glycans containing β1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly <I>N</I>-glycans, we found complex type <I>N</I>-glycans bearing both galactose and <I>N</I>-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found <I>N</I>-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-6 (Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-3) Manβ1-4GlcNAcβ1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the <I>N</I>-glycan moiety of eucaryotic glycoproteins.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(10), 2583-2587, 2006-10-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry