Quantification of Cyanamide Contents in Herbaceous Plants
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Cyanamide (NH<SUB>2</SUB>CN) is found in nature, although it has long been recognized as an industrial product. Distribution of cyanamide in the plant kingdom was investigated using a direct quantitative determination method to detect and measure cyanamide by stable isotope dilution gas chromatography–mass spectrometry (the SID–GC–MS method). The SID–GC–MS method proved to be a robust way to quantify cyanamide contents in the extracts of 101 species of herbaceous plants. The average recovery of cyanamide from all plants tested was 55.6±20.3%. <I>Vicia villosa</I> and <I>V. cracca</I> contained cyanamide at 369–498 μg/gFW and 3,460–3,579 μg/gFW respectively, while the other 99 species contained no detectable cyanamide (<1 μg/gFW). This result suggests that distribution of cyanamide in the plant kingdom is limited and uneven.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(9), 2310-2312, 2007-09-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry