Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine N-Methyltransferase Gene
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Phosphoethanolamine <I>N</I>-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5′ untranslated region (UTR) of several <I>PEAMT</I> genes was found to contain an upstream open reading frame (uORF). We generated transgenic <I>Arabidopsis</I> calli that expressed a chimeric gene constructed by fusing the 5′ UTR of the <I>Arabidopsis PEAMT</I> gene (<I>AtNMT1</I>) upstream of the β-glucuronidase gene. The <I>AtNMT1</I> uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream β-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(9), 2330-2334, 2007-09-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry