Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine N-Methyltransferase Gene

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Phosphoethanolamine <I>N</I>-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5′ untranslated region (UTR) of several <I>PEAMT</I> genes was found to contain an upstream open reading frame (uORF). We generated transgenic <I>Arabidopsis</I> calli that expressed a chimeric gene constructed by fusing the 5′ UTR of the <I>Arabidopsis PEAMT</I> gene (<I>AtNMT1</I>) upstream of the β-glucuronidase gene. The <I>AtNMT1</I> uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream β-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(9), 2330-2334, 2007-09-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018527684
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    09168451
  • NDL 記事登録ID
    8073928
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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