Advanced NMR Approaches for a Detailed Structure Analysis of Natural Products

• FUKUSHI Eri

  Graduate School of Agriculture, Hokkaido University

Abstract

Some new nuclear magnetic resonance (NMR) approaches to elucidate chemical structures, which have not been determined by routine NMR methods, are presented. Selective detection of methine (CH), methylene (CH₂), or methyl (CH₃) signals in each subspectrum by editing NMR methods was utilized to reduce the complexity in crowded spectra. It also increased the peak separation and enhanced the sensitivity by limiting the measuring area of the 2D spectra. Several 2D methods to measure ^2,3J_CH values, which are useful for stereochemical assignment are then introduced. To determine the structure of a highly hydrogen-deficient molecule, efficient correlation methods for long-range ¹³C–¹³C coupling and ¹H-¹⁵N HMBC are also described.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (8), 1803-1812, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• editing NMR
• E-HSQC
• E-HSQC-ROESY
• DEPT C–C relay
• natural product

Details

• CRID: 1390001206479555584
• NII Article ID: 10018527703
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.50663
• ISSN: 13476947
• ISSN: 09168451
• HANDLE: 2115/14726
• NDL BIB ID: 8040070
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8040070
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.50663
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed