Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses

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rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.

<I>rcd1</I> is a mutant of <I>Arabidopsis thaliana</I> that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. <I>rcd1-2</I> is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The <I>rcd1-2</I> mRNA level does not change significantly with the mutation. Since overexpression of <I>rcd1-1</I> cDNA has been shown to bring about an <I>rcd1</I>-like phenotype, we created and examined the overexpression lines of <I>RCD1</I> by the use of the cauliflower mosaic virus <I>35S</I> promoter. The transgenic lines exhibited a weak <I>rcd1</I>-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant <I>rcd1-2</I> phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.

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  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(8), 1827-1831, 2006-08-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018527856
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8040102
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  IR  J-STAGE 
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