Microbial Production of Optically Active β-Phenylalanine through Stereoselective Degradation of Racemic β-Phenylalanine

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The ability to produce (<I>R</I>)- or (<I>S</I>)-β-phenylalanine from racemic β-phenylalanine through stereoselective degradation was screened for. <I>Variovorax</I> sp. JH2 and <I>Arthrobacter</I> sp. the faculty of Agriculture, Kyoto University (AKU) 638 were found to be potential catalysts for (<I>R</I>)- and (<I>S</I>)-β-phenylalanine production respectively. On 192 h cultivation of <I>Variovorax</I> sp. in medium containing 1.0% (w/v) racemic β-phenylalanine, 0.46% (w/v) (<I>R</I>)-β-phenylalanine with an enantiomeric purity of 99% <I>e.e.</I> was obtained. The initial step of the (<I>S</I>)-isomer degradation was stereoselective transamination. On 312 h cultivation of <I>Arthrobacter</I> sp. in medium containing 1.0% (w/v) racemic β-phenylalanine, 0.51% (w/v) (<I>R</I>)-β-phenylalanine with an enantiomeric purity of 90% <I>e.e.</I> was obtained. The initial step of the (<I>R</I>)-isomer degradation was supposed to be oxidative deamination. Resting cell reaction with vigorous shaking, with cells of <I>Arthrobacter</I> sp. as the catalyst, resulted in production of 0.49% (w/v) of (<I>S</I>)-β-phenylalanine with an enantiomeric purity of 99% <I>e.e.</I> from 1.0% (w/v) racemic β-phenylalanine in 45 h.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(8), 1941-1946, 2006-08-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018528270
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    8040453
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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